The Open Protein Structure Annotation Network
PDB Keyword


    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of Tat-interacting protein 30 kDa (HIV-1 tat interactive protein 2, 30 kDa Homolog) (Human) (16924205) from Mus musculus at 2.30 A resolution. To be published
    Site JCSG
    PDB Id 2fmu Target Id 358966
    Molecular Characteristics
    Source Mus musculus
    Alias Ids TPS1409,16924205 Molecular Weight 26222.83 Da.
    Residues 236 Isoelectric Point 8.95
    Sequence lpklredfkmqnksvfilgasgetgkvllkeilgqnlfskvtligrrkltfeeeayknvnqevvdfekl dvyasafqghdvgfcclgttrskagaegfvrvdrdyvlksaelakaggckhfnllssrgadksssflyl qvkgeveakveelkfdrlsvfrpgvllcdrqesrpgewlarkffgslpdswasgyavpvvtvvramlnn lvspssgqmellenkailhlgkdrdvpkl
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 1
    Resolution (Å) 2.30 Rfree 0.265
    Matthews' coefficent 2.33 Rfactor 0.203
    Waters 49 Solvent Content 47.10

    Ligand Information



    Protein Summary

    The gene Htatip2 from Mus musculus encodes the protein NP_058561, a homologue of human HIV-1 TAT interactive protein TIP30 (PDB ID: 2BKA).  The protein structure represents an NAD binding domain from PF01118, usually found in semialdehyde dehydrogenases.  The 2fmu structure belongs to the class of alpha and beta proteins (a+b) and reveals NAD(P)-binding Rossmann fold type SCOP51734.  

    The human homologue of the protein exhibits pro-apoptotic and anti-metastatic properties.  Although TIP30 has semialdehyde dehydrogenase fold type and is able to specifically bind NADPH, it does not exhibit relevant enzymatic activity [Ref].  The mutagenesis studies, done on the conserved Tyr-Lys-Ser NADPH binding motif, suggest that NADPH binding is essential for its biological activity.  These proteins provide an example of the adaptation of a metabolic enzyme fold to include a regulatory role.  This phenomenon has been also observed in the case of NADH-binding co-repressor CtBP [Ref].

    Ligand Summary





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