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    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary

    Title Crystal structure of a putative CN hydrolase from yeast. Proteins 52 283-291 2003
    Site NYSGXRC
    PDB Id 1f89 Target Id NYSGXRC-P018
    Molecular Characteristics
    Source Saccharomyces cerevisiae
    Alias Ids TPS8063,P49954 Molecular Weight 32547.48 Da.
    Residues 291 Isoelectric Point 7.09
    Sequence msaskilsqkikvalvqlsgsspdkmanlqraatfieramkeqpdtklvvlpecfnspystdqfrkyse vinpkepstsvqflsnlankfkiilvggtipeldpktdkiyntsiifnedgklidkhrkvhlfdvdipn gisfhesetlspgeksttidtkygkfgvgicydmrfpelamlsarkgafamiypsafntvtgplhwhll arsravdnqvyvmlcsparnlqssyhayghsivvdprgkivaeagegeeiiyaeldpeviesfrqavpl tkqrrfdvysdvnah
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 2
    Resolution (Å) 2.40 Rfree 0.258
    Matthews' coefficent 2.26 Rfactor 0.229
    Waters 117 Solvent Content 49.00

    Ligand Information


    Google Scholar output for 1f89
    1. Catalysis in the nitrilase superfamily
    C Brenner - Current opinion in structural biology, 2002 - Elsevier
    2. Structural genomics: a pipeline for providing structures for the biologist
    MR Chance, AR Bresnick, SK Burley, JS Jiang - Protein , 2002 - Wiley Online Library
    3. Statistical analysis and prediction of proteinprotein interfaces
    AJ Bordner, R Abagyan - Proteins: Structure, Function, and , 2005 - Wiley Online Library
    4. Post_translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form
    RN Thuku, BW Weber, A Varsani, BT Sewell - FEBS Journal, 2007 - Wiley Online Library
    5. Microbial nitrilases: versatile, spiral forming, industrial enzymes
    RN Thuku, D Brady, MJ Benedik - Journal of applied , 2009 - Wiley Online Library
    6. Crystal structure of a putative CN hydrolase from yeast
    D Kumaran, S Eswaramoorthy - Proteins: Structure, , 2003 - Wiley Online Library
    7. An iterative knowledge_based scoring function for proteinprotein recognition
    SY Huang, X Zou - Proteins: Structure, Function, and , 2008 - Wiley Online Library
    8. Crystal structure of hypothetical protein PH0642 from Pyrococcus horikoshii at 1.6 resolution
    N Sakai, Y Tajika, M Yao, N Watanabe - Proteins: Structure, , 2004 - Wiley Online Library
    9. Variation among genome sequences of H37Rv strains of Mycobacterium tuberculosis from multiple laboratories
    TR Ioerger, Y Feng, K Ganesula, X Chen - Journal of , 2010 - Am Soc Microbiol
    10. Structure of amidase from Pseudomonas aeruginosa showing a trapped acyl transfer reaction intermediate state
    J Andrade, A Karmali, MA Carrondo - Journal of Biological , 2007 - ASBMB
    11. Motif-based protein ranking by network propagation
    R Kuang, J Weston, WS Noble, C Leslie - Bioinformatics, 2005 - Oxford Univ Press
    12. Influence of different carboxy-terminal mutations on the substrate-, reaction-and enantiospecificity of the arylacetonitrilase from Pseudomonas fluorescens EBC191
    C Kiziak, J Klein, A Stolz - Protein Engineering Design and , 2007 - Oxford Univ Press
    13. Combinatorial docking approach for structure prediction of large proteins and multi-molecular assemblies
    Y Inbar, H Benyamini, R Nussinov, HJ Wolfson - Physical biology, 2005 - iopscience.iop.org
    14. The Crystal Structure of _-Alanine Synthase from Drosophila melanogaster Reveals a Homooctameric Helical Turn-Like Assembly
    S Lundgren, B Lohkamp, B Andersen, J Pikur - Journal of molecular , 2008 - Elsevier
    15. Structure of an aliphatic amidase from Geobacillus pallidus RAPc8
    SW Kimani, VB Agarkar, DA Cowan - Section D: Biological , 2007 - scripts.iucr.org
    16. Oligomeric structure of nitrilases: effect of mutating interfacial residues on activity
    BT Sewell, RN Thuku, X Zhang - Annals of the New York , 2005 - Wiley Online Library
    17. Functional Proteomic and Structural Insights into Molecular Recognition in the Nitrilase Family Enzymes
    KT Barglow, KS Saikatendu, MH Bracey, R Huey - Biochemistry, 2008 - ACS Publications
    18. Investigative mining of sequence data for novel enzymes: A case study with nitrilases
    JL Seffernick, SK Samanta, TM Louie, LP Wackett - Journal of , 2009 - Elsevier
    19. The quaternary structure of the amidase from Geobacillus pallidus RAPc8 is revealed by its crystal packing
    VB Agarkar, SW Kimani, DA Cowan - Section F: Structural , 2006 - scripts.iucr.org
    20. The crystal structure of XC1258 from Xanthomonas campestris: a putative procaryotic Nit protein with an arsenic adduct in the active site
    KH Chin, YD Tsai, NL Chan, KF Huang - Proteins: Structure, , 2007 - Wiley Online Library
    21. Directed evolution of Alcaligenes faecalis nitrilase
    U Schreiner, B Hecher, S Obrowsky, K Waich - Enzyme and Microbial , 2010 - Elsevier
    22. Crystallographic analysis of a thermoactive nitrilase
    JE Raczynska, CE Vorgias, G Antranikian - Journal of structural , 2011 - Elsevier
    23. Improved Fitness of Arabidopsis thaliana Nitrilase 2
    U Schreiner, G Steinkellner, JD Rozzell - , 2010 - Wiley Online Library
    24. Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10
    O Kaplan, K Bezouka, O Plhal, R Ettrich - BMC , 2011 - biomedcentral.com
    25. Structural and biochemical characterization of a nitrilase from the thermophilic bacterium, Geobacillus pallidus RAPc8
    DS Williamson, KC Dent, BW Weber, A Varsani - Applied microbiology , 2010 - Springer
    26. Patch prediction of protein interaction sites: validation of a scoring function for an online server
    S Jones, Y Mukarami - Bioinformatics Research and Development, 2007 - Springer
    27. Cloning, crystallization and preliminary X-ray study of XC1258, a CN-hydrolase superfamily protein from Xanthomonas campestris
    YD Tsai, KH Chin, HL Shr, FP Gao, PC Lyu - Section F: Structural , 2006 - scripts.iucr.org
    28. Biochemical and Mutational Studies of the Bacillus cereus CECT 5050T Formamidase Support the Existence of a CEEK Tetrad in Several Members of the Nitrilase
    P Soriano-Maldonado, AI Martnez-Gmez - Applied and , 2011 - Am Soc Microbiol
    29. _-alanine synthase: one reaction, two folds and mechanisms
    S Lundgren - 2007 - diss.kib.ki.se
    30. Supplementary Discussion
    ED Levy, EB Erba, CV Robinson, SA Teichmann - moult.ibbr.umd.edu
    31. Modeling Substrate-Enzyme Interactions in Fungal Hydrolases
    N Kulik, N Hrady - theses.cz

    Protein Summary

    Ligand Summary




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